Effect of a κB decoy oligonucleotide on CD69 expression (A) and the levels of NF-κB/Rel nuclear complexes (B) in CD40-stimulated B-CLL cells. (A) B-CLL cells (1 × 10⁶/mL) were stimulated with anti-CD40 MoAb (10 μg/mL) in the absence or the presence of the 5-μmol/L κB decoy or scrambled oligonucleotide. After 3 days the cells were collected, washed, incubated with CD69 PE and CD19 FITC, and analyzed by FACScan. The proportion of the CD19⁺ cells in the different cultures was 74.8 ± 2.4. (□), Medium; (▪), anti-CD40 MoAb. (B) B-CLL cells (1 × 10⁶/mL) were incubated in 10% FCS-RPMI 1640 medium (a), and with anti-CD40 MoAb (10 μg/mL) in the absence (b) or presence of the 5-μmol/L κB decoy (c) or scrambled (d) oligonucleotide. After 20 hours, nuclear extracts were obtained and incubated with the ³²P-labeled κB oligonucleotide. The DNA-protein complexes were analyzed by EMSA.