Anti-TNF–treated GTS cultures produce distinct progeny in methylcellulose and liquid cultures, compared with GTS cultures. GTS cultures yielded typical DC colonies around day 12 in methylcellulose (a) and adherent DC clusters in liquid cultures (c). In methylcellulose, anti-TNF–treated GTS cultures produced large CFUs containing small round cells and lacking typical DCs (b). The liquid anti-TNF–treated cultures (d) contained predominantly nonadherent cells. Original magnification × 20.