Kinetics of appearance of ER-MP58⁺ and ER-MP20⁺ cells in regions 1 and 2. At various times after TM injection, peritoneal cells from region 1 (solid symbols) and region 2 (open symbols) (see Fig 3) were monitored for ER-MP58 (squares), ER-MP20 (circles), and ER-MP12 (triangles) by single-color flow cytometry (see the Materials and Methods). In (A), the data are expressed as a percentage of the number of cells in the respective region, whereas in (B) the data are expressed as cell numbers in each region per peritoneal cavity. Data are the means ± range of variation from 2 experiments (8 mice/time point). A partial kinetic analysis was also performed another 8 times. When error bars are not presented, the errors are smaller than the size of the symbols. Because of their low incidence, the numbers of ER-MP12⁺ cells elicited by TM in both regions were not presented in (B).