ER-MP20⁺ peritoneal cells in regions 1 and 2 are ER-MP58⁺ and Mac-1⁺. At 16 hours after TM injection, peritoneal cells from region 1 (A and B) and region 2 (C and D) (see Fig 3) were stained for both ER-MP20 and ER-MP58 (A and C) and were stained for both ER-MP20 and Mac-1 (B and D); two-color flow cytometric analysis was then performed (see the Materials and Methods). The percentages of cells are presented in the top right quadrant. There are fewer cells in region 1 due to the macrophage disappearance reaction (see Fig 3). Data are from a representative experiment (8 mice). The experiment was repeated twice more.