Fig. 2.
![Fig. 2. Translocation of PKC isozymes in basophils stimulated with PMA. Basophils (purity = 80% ± 3%, n = 4) were incubated with or without PMA (100 ng/mL) for 15 minutes. The membrane and cytosol fractions were subjected to Western blot analysis and the band intensities were quantified by digital imaging. The top panel shows a representative Western blots from one experiment and the lower panel shows the amount of each isozyme in the cytosol or membrane expressed as a percentage of either component divided by the sum of both components [eg, cytoscol/(cytosol + membrane)]. Data are presented as mean ± SEM for four experiments. * P < .05, ***P < .001 (paired t-test).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/4/10.1182_blood.v92.4.1206/5/blod41601002w.jpeg?Expires=1735545375&Signature=I9Qa1cC4tHikKAXnZu~J87nZE35ZpXg2-yLPWdcESauYiOmm2azgMrytOhnjd51jorZRMJ5RrQ7ltnlKXLegufXq9lLT7EPAd1NbzlIJYszXr1oDsGelr2te2M~3blsfvgu74Lu1ep2gsXxC9va-WaFhG0ekBX3lSBGxNC4Z3uFZFgVx3nKMzalwK61wtM2ewtIhMusBTYqqjKVttBd-ImUJPy6agbrUR1Vqbo4k07BMsiiAcik8uedEZ8Ydx7M~4nn8jVclYknB~2aCXcSidCjTlUPwJbBYYIY-1J1ttNLGpisPB-4VD3Cib54c0a8sCYDmzyJbmLoLLH-b9iv70A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Translocation of PKC isozymes in basophils stimulated with PMA. Basophils (purity = 80% ± 3%, n = 4) were incubated with or without PMA (100 ng/mL) for 15 minutes. The membrane and cytosol fractions were subjected to Western blot analysis and the band intensities were quantified by digital imaging. The top panel shows a representative Western blots from one experiment and the lower panel shows the amount of each isozyme in the cytosol or membrane expressed as a percentage of either component divided by the sum of both components [eg, cytoscol/(cytosol + membrane)]. Data are presented as mean ± SEM for four experiments. * P < .05, ***P < .001 (paired t-test).
