PKC translocation in basophils stimulated with FMLP. Basophils (purity: 73%) were stimulated with 1 μmol/L FMLP and after the times indicated the cells were harvested, membrane and cytosol fractions were prepared, analyzed by Western blotting, and the bands quantified by digital imaging. The data shows one of two experiments. The data on the lower panel is expressed as the amount of membrane-associated PKC as a percentage of the summed band intensities for membrane + cytosol. PKC βI (○), βII (□), δ (▪), ε (•), and histamine (▴).