PKC translocation in basophils stimulated with anti-IgE antibody. Basophils (purity of 71%) were stimulated with 0.2 μg/mL of anti-IgE antibody and after the times indicated the cells were harvested, membrane and cytosol fractions were prepared, analyzed by Western blotting and the bands quantified by digital imaging. The results shown in the top half of the figure are from one of three experiments, the one with the highest histamine release (71%), and the bottom half of the figure shows the average of three experiments (average histamine release was 34%).