Binding of CD164 MoAbs to stable FDCP-1 cells expressing CD164. FDCP-1 cells stably transfected with CD164 cDNA were labeled with the N6B6, 67D2, 105A5, or 103B2/9E10 MoAbs or with irrelevant first MoAbs of the same isotype. The reaction was developed with FITC-conjugated anti-mouse Ig antibody as detailed in Materials and Methods, and the median fluorescence values were determined after FACSCalibur analysis. The results of one of three experiments are shown. The IgG negative control histogram contains the second FITC antibody only. The median fluorescence intensity values for the negative isotype matched controls were: no first MoAb = 3.4; IgG3 = 3.4; IgG2a = 3.4; IgM = 3.4; and IgG1 = 3.3. The median fluorescence for each CD164 MoAb was: 103B2/9E10 = 45.32; 105A5 = 26.42; 67D2 = 138.24; and N6B6 = 121.37.
