Differential expression of CD164 epitopes on peripheral blood lymphocytes, monocytes, and granulocytes. Ficoll separated cells were stained with CD164-specific MoAbs, 103B2/9E10, 105A5, 67D2, and N6B6, selected for lymphocytes, monocytes, or granulocytes on the basis of forward and side scatter parameters and analyzed on a FACSCalibur flow cytometer. Representative histograms from one of three independent experiments are shown. Median fluorescence values after subtraction of the median fluorescence value of the negative isotype-matched control for each population in this experiment are as follows: for the lymphocytes: N6B6 = 8.01; 67D2 = 6.49; 103B2/9E10 = 5.01; 105A5 = 1.37; for the monocytes: N6B6 = 35.05; 67D2 = 58.51; 103B2/9E10 = 53.50; 105A5 = 2.09; for the granulocytes: N6B6 = 2.41; 67D2 = 4.41; 103B2/9E10 = 2.59; 105A5 = 0.31. Mature non-nucleated glycophorin-A⁺ erythrocytes did not stain with any of the CD164 MoAbs.