Fig. 5.
CD34−CD164(103B2/9E10 epitope)+ bone marrow and cord blood cells contain nucleated erythroid cells. Ficoll separated bone marrow or cord blood mononuclear cells were labeled with 103B2/9E10 together with the PE-antibody conjugates indicated on the dot plots before staining with FITC-F(ab)2 goat anti-mouse IgG3 and flow cytometric analyses. Plots are two-color displays of cells gated on the lymphoid/erythroid/blast cell gate in Fig 3. The arbitrary gates shown on the dot plots were determined with negative control isotype-matched irrelevant antibodies as described in Materials and Methods.
