TPA-induced differentiation of CMK cells on day course study. After addition of 20 nmol/L TPA to CMK cells, whole cell lysates were subjected to immunoblotting analyses with (A) anti-integrin IIb MoAb, (B) anti-integrin β3 MoAb, or (C) anti-Syk polyclonal antibody. In each lane, 30 μg protein of cell lysates was loaded. The cell lysates were next immunoprecipitated with (D) antiphosphotyrosine MoAb (PY20) or (E) anti-FAK polyclonal antibody. Immunoblotting analyses were performed with antiphosphotyrosine MoAb (4G10) as described in the Materials and Methods. In (D), arrows indicate the positions of the tyrosine-phosphorylated protein bands. In (E), the blots were stripped and reprobed with the anti-FAK polyclonal antibody. Results are representative of 3 experiments.