Fig. 3.
Western blot analysis of 32D cell lysates. (A) 32D-neo, 32D-c-myc, and 32D-max cells were electroporated with either the linearized pMEP4-Bcl-2 expression vector or the empty pMEP4 parental vector. Stable clones were selected in hygromycin and pooled. From each cell line 50 μg of total cell lysate was subjected to SDS-PAGE and Western blotting using an anti–Bcl-2 antibody. (B) 32D-neo, 32D-c-myc, and 32D-max cell lines were stably transfected with either the pAPuro-Bcl-XL vector or the pAPuro parental vector. SDS-PAGE and Western blotting were performed as described in (A) except that an anti–Bcl-X antibody was used.
