Effects of Bcl-2 and Bcl-XL on drug-mediated apoptosis in 32D cell lines. 32D-neo, 32D-c-myc, and 32D-max cells were stably transfected with Bcl-2 or Bcl-XL expression plasmids or with the respective empty parental vectors. The resulting pooled clones were then tested for their ability to undergo apoptosis in response to treatment with either ADR or CPT. (A) Effects of ectopic Bcl-2 expression on the response to ADR treatment. The results shown (±1 SE) are those obtained after a 42-hour exposure to the indicated concentrations of the drug. (B) Effects of ectopic Bcl-2 expression in response to a 48-hour exposure to the indicated concentrations of CPT. (C) Effects of ectopic Bcl-XL expression in response to a 48-hour exposure to the indicated concentrations of CPT. The results shown are representative of three experiments ±1 SE.
