Detection of nuclear proteins interacting with the −142 to −89 NRE of the vWF gene by mobility shift assays. (A) 5′-end of the sequence of the −142 to −89 wild-type probe and of the −142 to −89 mutant probe. The mutated basepairs are underlined. (B) The restriction enzyme fragments were labeled and incubated with nuclear extracts from HUVECs or HeLa cells before electrophoresis as described in the Materials and Methods. The competitors were wild-type fragment (WT), mutant fragment (Mut), ROR, and MLP oligonucleotides at the indicated molar excess. Arrows designate the specific retarded complexes as described in the text.