The high-affinity nuclear protein binding site in the vWF gene promoter NRE is located inside the sequence NRE1 (−142 to −114). The double-stranded oligonucleotide NRE (−142 to −89) was labeled, incubated with nuclear extracts from HUVECs, and analyzed as described in the Materials and Methods. Unlabeled double-standed competitors NRE, NRE1 (−142 to −114) and NRE2 (−113 to −85) were included at 50-, 100-, and 200-fold molar excess. The major complex 1 is indicated by an arrow.