Effect of CD36 iodination on the cytoadherence of PEs. Adhesion assay was as in Fig 1 with all assays performed at pH 6.8. Iodination was performed by subjecting the transfected COS-7 cells to no additions (a); addition of 10 mmol/L NaI in PBS (b); addition of an iodobead (c); or the addition of both 10 mmol/L NaI and an iodobead in PBS (d). Results represent the mean with standard deviation indicated by error bars. Similar results were obtained in three independent experiments. Values indicated are for (A) human CD36 and F97Y (human CD36 with residue 97 substitute for tyrosine) and for (B) human CD36, murine CD36, rat CD36, H242Y (human CD36 with residue 242 substituted for tyrosine), and Y242H (rat CD36 with residue 242 substituted for histidine). Iodination significantly reduces the level of cytoadherence to murine CD36, rat CD36, and H242Y (P < .01,t-test).