Fig. 4.
Absence of production of O2− by PU.1-deficient neutrophils in response to activation by PMA. O2− production was assessed in normal (▨,▪) and PU.1-deficient (▧,□) neutrophils by colorimetric detection of cytochrome c reduction. Baseline (A) and post-PMA stimulation (B) levels of O2− were measured. Samples were run in triplicate and the results of a representative experiment are presented as mean ± SD.
