Conservation of Gly₂₇₉ and TM9 in Rh50 homologues from three species. Comparison of amino acid sequences (equivalent to residues 270-315 in humans) encoded by exon 6 of Rh50 homologues. The Gly₂₇₉→Glu (G279E) mutation found in Rh_null (YT) is indicated by an arrow. h,Homo sapiens; m, Mus musculus; c,Celegans; and g, Geodia cydonium. Identical amino acids are indicated by colons. Note that exon 6 is identical in size between the human and mouse Rh50, but splits into two exons inC elegans Rh-1. Predicted TM9 and TM10 are boxed. The sequences for human Rh30s, RhCE (upper), and RhD (lower) are also aligned. Note the two consecutive glycine residues (G₂₇₉G₂₈₀) and their flanking amino acids are conserved in the Rh family of proteins. In the TM9 of Rh50 homologues, each pair of the three substitutions (A275S, V283I, and T285S) is similar in nature, rendering a 100% similarity between C elegans and Homo sapiens.