Functional analysis of the wild-type and mutant (−94 C to G) factor VII promoters. One hundred eighty-six base pair fragments of wild-type or mutant (MT94) human factor VII promoter sequence were inserted into the hGH reporter vector and used to transiently transfect HepG2 cells. The data were corrected as described in Materials and Methods, and the level of hGH expression from reporter plasmid containing the wild-type sequence was considered 100%. Relative to this, the level of hGH expression from the MT94 sequence was 5.8 ± 2.2% (1 SD). The data shown were obtained from three experiments in which a total of 14 dishes were transfected with each expression vector.