Fig. 3.
Fig. 3. Interactions with an Sp1 consensus sequence and human Sp1 protein. (A) Unlabeled wild-type (WT) but not mutant (MT94) human factor VII sequence competes for Sp1 binding with radiolabeled Sp1 consensus sequence. Lane 1 shows binding of radiolabeled Sp1 consensus probe by HeLa nuclear extract in the absence of unlabeled competitor sequences. Lanes 2 through 4 show Sp1 consensus probe binding with HeLa nuclear extract in the presence of 1,000× concentrations of unlabeled competitor Sp1 consensus sequence (lane 2), WT factor VII sequence (−108 to −84; lane 3), and MT factor VII–deficient patient sequence (−108 to −84; lane 4). (B) WT but not MT94 factor VII–deficient patient sequence binds to the transcription factor Sp1. Binding of radiolabeled oligonucleotides with recombinant human Sp1 is shown with radiolabeled Sp1 consensus sequence (lane 1) and WT factor VII sequence (lane 2) but not with MT factor VII–deficient patient sequence (lane 3).

Interactions with an Sp1 consensus sequence and human Sp1 protein. (A) Unlabeled wild-type (WT) but not mutant (MT94) human factor VII sequence competes for Sp1 binding with radiolabeled Sp1 consensus sequence. Lane 1 shows binding of radiolabeled Sp1 consensus probe by HeLa nuclear extract in the absence of unlabeled competitor sequences. Lanes 2 through 4 show Sp1 consensus probe binding with HeLa nuclear extract in the presence of 1,000× concentrations of unlabeled competitor Sp1 consensus sequence (lane 2), WT factor VII sequence (−108 to −84; lane 3), and MT factor VII–deficient patient sequence (−108 to −84; lane 4). (B) WT but not MT94 factor VII–deficient patient sequence binds to the transcription factor Sp1. Binding of radiolabeled oligonucleotides with recombinant human Sp1 is shown with radiolabeled Sp1 consensus sequence (lane 1) and WT factor VII sequence (lane 2) but not with MT factor VII–deficient patient sequence (lane 3).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal