American Society of Hematology

Fig. 2.

$Fig. 2. Southern blot analysis of LEUK1 and normal control DNA probed with a MOZ cDNA probe. High molecular weight DNA was digested to completion with Hind 3, Bgl 2, or Stu 1; size-fractionated; blotted; and hybridized to a probe that extends from bp 2549 to 3663 in the MOZ cDNA. The blot was washed in 0.1× SSC at 65°C for 30 minutes. Lanes 1, 4, and 8 contain DNA from LEUK1 cells; lanes 2, 5, and 9 contain DNA from normal volunteer no. 1; lanes 3, 6, and 10 contain DNA from normal volunteer no. 2; and lanes 7 and 11 contain DNA from normal volunteer no. 3. The arrows indicate the positions of the rearranged restriction fragments in LEUK1 DNA. Normal volunteer no. 1 has restriction fragment length polymorphisms after Hind 3 and Stu 1 digestion (lanes 2 and 9).$

Southern blot analysis of LEUK1 and normal control DNA probed with a MOZ cDNA probe. High molecular weight DNA was digested to completion with Hind 3, Bgl 2, or Stu 1; size-fractionated; blotted; and hybridized to a probe that extends from bp 2549 to 3663 in the MOZ cDNA. The blot was washed in 0.1× SSC at 65°C for 30 minutes. Lanes 1, 4, and 8 contain DNA from LEUK1 cells; lanes 2, 5, and 9 contain DNA from normal volunteer no. 1; lanes 3, 6, and 10 contain DNA from normal volunteer no. 2; and lanes 7 and 11 contain DNA from normal volunteer no. 3. The arrows indicate the positions of the rearranged restriction fragments in LEUK1 DNA. Normal volunteer no. 1 has restriction fragment length polymorphisms after Hind 3 and Stu 1 digestion (lanes 2 and 9).

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