Adhesion of neutrophils to E3-ICAM transfectants. Neutrophils (3 × 10⁶ cells/mL) were mixed with either E3-ICAM–transfected cells or B78H1 parent cells (6 × 10⁶ cells/mL), stimulated with 1 μmol/L FMLP, and sheared at a shear rate of 200 s⁻¹. The percentage of neutrophils in heterotypic aggregates 10 seconds after the application of shear is compared on addition of a panel of blocking antibodies at saturation concentration: R3.1 Fab (to LFA-1), h60.1 (to Mac-1), and R6.5 Fab (to ICAM-1). *The fifth bar represents experiments where R6.5 Fab was added to bind 50% of the ICAM-1 receptors on E3-ICAM cells.^#Not statistically different. Error bars represent SEM from at least three independent experiments.