Cation exchange chromatography of pulse-radiolabeled globin chains in an HbE/β thalassemic (Cd16) at 37°C, showing globin binding to the membrane 30 minutes, 2 hours, and 12 hours after removal of the radiolabeled leucine. The arrows show the position of each globin peak as determined by the separation of the nonradiolabeled protein. The vertical axis shows counts per minute (cpm). The initial peak of  globin is lost after 12 hours.