Summary of flow cytometry gating procedures for the study of GPI-linked antigens (CD48 or CD59) by peripheral blood T cells, B cells, and NK cells. Plot (A) shows a region (R1) drawn around CD3⁺ cells with low side scatter characteristics; plot (B) shows a region (R1) drawn around CD19⁺ cells with low side scatter characteristics; plots (C and D) show the gating procedure for NK cells. An initial region or anchor gate (R2) is set around CD7⁺CD3⁻ cells. This region is then applied to an FSC versus SSC plot and a second region (R3) drawn around the lymphocytes with low FSC and SSC characteristics. Only events (cells) meeting these two criteria are collected and analyzed.