Preparation of recombinant baculovirus transfer vectors for infection of SF9 cells. (A) Restriction sites and stop codons used to truncate IIb/β3 are shown. H/L denotes IIb heavy-light chain junction; TM indicates transmembrane domains. (B) Truncated IIb/β3 cDNAs were inserted into baculovirus transfer vector p2Bac under control of the baculovirus p10 and polyhedron (Pol) promoters, respectively, as shown. Indicated restriction enzyme sites are E (EcoRI), H (HindIII), Xm (Xma I), and Xb (Xba I).