(A) The sequence surrounding the ZNF198-FGFR1 fusion. Proline 913 of ZNF198 is joined to valine 429 of FGFR1.39FGFR1 sequence is underlined. (B) RT-PCR analysis showing the presence of ZNF198-FGFR1 fusion transcripts (578 bp) in patients P.R., J.B., and E.C. but not three patients with CML. Reciprocal FGFR1-ZNF198 transcripts were not detected in any individual. M, 123-bp ladder. (C) (i) Expression of normal ZNF198 (527 bp) and normal FGFR1 (534 bp) in patients P.R., J.B., and E.C. (ii) RT-PCR using (A) primers DET22 + DET26 (564 bp), (B) DET17 + DET26 (395 bp), and (C) DET17 + DET22 + DET26 (564 bp).