Flow cytometric analysis of BAH-1 surface protein expression in a DAMI megakaryocytic cell line. Antigen expression was evaluated by FACS staining using BAH-1 and Mpl-R MoAbs (commercially available from Genzyme, MA), CD61 or control MoAb on DAMI (A) and Jurkat T cells (B). X653, supernatant from the mouse myeloma cell line used in cell fusion; 12R, a control nonrelevant MoAb (1:1,000); CD61, a positive control for megakaryocytic cells; BAH-1 Asc, ascites of BAH-1 (1:1,000 dilution); BAH-1 Sup, BAH-1 supernatant (1:1,000 dilution).