Fig. 1.
Detection of vector sequences in peripheral blood from baboons T95096 (A) and T94397 (B). Animals were transplanted with CD34-enriched marrow cells transduced by 48 hours of cocultivation (LNX) or on CH-296 (LN) in the presence of IL-3, IL-6, and SCF without protamine sulfate (A) and with protamine sulfate (B). PCR was performed to quantitate LNX and LN vector amounts. LNX versus LN. Standards consist of single-vector copy HT1080/LN and HT1080/LNX DNA mixed at a 1:1 ratio and then mixed with normal baboon DNA in a log dilution series. PB, peripheral blood.