Detection of vector sequences in peripheral blood and marrow from baboon T94433 transplanted with CD34-enriched marrow cells transduced by 72 hours of cocultivation (LNX) or over CH-296 (LN) both in the presence of IL-6, SCF, FLT3L, MGDF, and protamine sulfate. (A) PCR analysis of amplified vector sequences, LNX versus LN. PB, peripheral blood; BM, bone marrow. (B) Percentage of vector-positive DNA measured by phosphorimage analysis of signal intensities for LN and LNX corrected for the amount of DNA as determined by actin PCR. (C) Southern blot analysis for the presence of vector sequences in DNA from peripheral blood. DNA was restricted with Sac I, which cuts both vectors outside the neo gene, and HindIII, which cuts the LNX vector only. The resulting fragments were 3,052 bp (LNL6), 2,370 bp (LN), and 1,932 bp and 464 bp (LNX). (D) Southern blot analysis of DNA from PB and BM restricted with Xba I, which cuts the vectors once in the LTR and therefore results in the full-length vector fragment only of integrated retrovirus. The respective fragments are 3,049 bp (LNL6), 2,369 bp (LN), and 2,394 bp (LNX). LN/LNX, both vectors are detected at the same place on the gel.