As2O3 and melarsoprol induce growth inhibition and apoptosis in a PML independent manner. Wild-type and PML−/− MEFs were incubated with the indicated concentrations of As2O3 (A) and melarsoprol (B) for 24 hours, and apoptotic cells were identified by in situ TdT. For each condition, at least 300 cells were counted. No differences in apoptosis and growth inhibition induced by As2O3 and melarsoprol between wild-type and PML−/− cells were found. For in vitro BM cultures (Materials and Methods), wild-type and PML−/− cells were incubated with As2O3 and melarsoprol at a concentration of 10−6 mol/L. At day 2 and day 6 CFU-E (C) and CFU-GM (D) were counted in triplicate. The data are expressed as a percentage of colony formation of arsenicals-treated versus untreated cells: untreated = 100%. The bars indicate the mean values ± SD from one representative experiment out of three.
