Analysis of expression of the proto-oncogenes c-myb and c-myb (A), MyD genes (B), and late genetic markers (C) before and after stimulation of M1 and M1Egr-1 cells with 1 and 50 ng/mL of IL-6. RNA was extracted from the cells at the indicated times after stimulation with IL-6. The RNA was resolved on formaldehyde-agarose gels and transferred to Durolon nylon membranes for Northern blot analysis. These data were generated using two RNA blots prepared at the same time from common RNA samples. Blots were stripped and reprobed as described in Materials and Methods.