Sickle RBCs do not adhere to known adhesive regions of laminin, murine EHS laminin, or human laminin-2. (A) Peptides representing known adhesive regions of laminin (GRGDSP, IKVAV, and YIGSR) were immobilized (1 mmol/L) and tested for their ability to support sickle RBC adhesion. These peptides did not support sickle RBC adhesion beyond nonspecific background levels. Adhesion to BSA and hLAM are shown as negative and positive controls, respectively. N = number of patients tested for each peptide. (B) SS RBCs were flowed across immobilized laminin-2 or mouse EHS laminin. Only background levels of sickle cells adhered to laminin-2 (hLAM-2) or mouse laminin (mLAM) compared with human placental laminin (hLAM). Both positive and negative controls are presented. Data shown represent the mean ± SD from six experiments involving 3 patients.