TMD4 mutations in Nramp2 do not alter localization to transferrin cycle endosomes. Epitope-tagged G185R, G184D, and D192GNramp2 cDNA mutant constructs were transfected into HEK293T cells and cells were treated as described in Fig 3A. All three mutant forms of Nramp2 show colocalization with Texas-red transferrin.