Fig. 5.
Binding of siglec-5 to polyacrylamide glycoconjugates in comparison with other siglecs. Fc-siglecs at 1 μg/mL were immobilized to plastic via anti-Fc antibody and biotinylated polyacrylamide (PAA) glycoconjugates linked either to 3′sialyllactose (2,3-PAA) or 6′sialyllactose (2,6-PAA) was added at the indicated concentrations. Unbound conjugate was washed off and binding detected with 125I-streptavidin. Data show means ± standard deviations of triplicates and are representative of four experiments performed.
