Fig. 8.
![Fig. 8. Representative two-dimensional tryptic phosphopeptide maps of TOPO IIβ protein from control (left) and RA-treated (right) HL-60 cells. Cells were metabolically labeled with [32P]-orthophosphoric acid, and lysates in RIPA buffer were immunoprecipitated with antiserum specific for TOPO IIβ. Peptides were separated horizontally by electrophoresis at pH 1.9 and vertically by chromatography as described in Materials and Methods. The sites that are differentially phosphorylated between control and RA-treated cells are identified by arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/8/10.1182_blood.v92.8.2863/5/blod42020008w.jpeg?Expires=1735847575&Signature=Lfpuet~xiD06BuwLkUAH3UQc9Fg5bfvf75ZsDODdaBL43A4lJ890kjRMOLAjvypQVDt797etkbm39YxhNBbr9zORi5EwV~4x24Orlf83UIt4neY4PpfXK1H75WqeXcoGShOW0Xv4DBNdbA0BZRw7IEIrlXcO~kKM9WPMYtTFb4IfHRjzDXijPRXE9KRYK5~hBwUKjsKWLvAfJiBxa5fZ1SyuJnQACndtqNCNIiGynYbyrEQSWjIA090ODt7fGcmgtRGZxTPwBxdD5lkUL42G7OPz-V9qMzIGwf7I9YS3juFAmV5M5UyFEDYlcXhX5vgRJ84oRGNohGQm0QHIh~THfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Representative two-dimensional tryptic phosphopeptide maps of TOPO IIβ protein from control (left) and RA-treated (right) HL-60 cells. Cells were metabolically labeled with [32P]-orthophosphoric acid, and lysates in RIPA buffer were immunoprecipitated with antiserum specific for TOPO IIβ. Peptides were separated horizontally by electrophoresis at pH 1.9 and vertically by chromatography as described in Materials and Methods. The sites that are differentially phosphorylated between control and RA-treated cells are identified by arrows.
