Southern blot analysis of PTEN gene. High-molecular-weight DNA was digested with HindIII, and the blot was hybridized with (top panel) the JL25 probe or (bottom panel) GAPDH to estimate DNA loading. CEM (lane 10) showed homozygous deletion of the PTEN gene. Lane 1, placental DNA; lane 2, SUDHL4; lane 3, SUDHL5; lane 4, SUDHL6; lane 5, SUDHL10; lane 6, NUDHL1; lane 7, KMS5; lane 8, KMS11; lane 9, Jim3; lane 10, CEM; lane 11, HUT102; lane 12, L428; lane 13, YT.