Examples of PCR-SSCP analysis of PTEN exons (A, exon 1; B, exon 3; C, exon 6; D, exon 7; E, exon 8). DNA was prepared and subjected to PCR-SSCP analysis. In each analysis, lane 1 contains control DNA (placental). Alterations are present in exon 1, lane 5 (primary LBCL), exon 3, lane 4 (SUDHL10), exon 6, lane 3 (PA682PB), exon 7, lane 5 (Jurkat), and exon 8, lanes 2, 11, and 16 (primary case 1706, Jim3, and primary case 1517, respectively).