Fig. 4.
Effect of interaction between human venous endothelial cells (VE cells) and K562 cells on secretion of endothelial IL-8 and apoptosis. K562 cells (5 × 104cells) were seeded with or without confluent VE cells in 24-well plates. Cell culture was boosted with or without anti–IL-8 antibody (5 μg/mL) every 24 hours. After 2 days, Wright-Giemsa staining was performed in 24-well plates (A). K562 cells and supernatants were collected, and MTT assay (B), TUNEL assay (C), and ELISA for IL-8 were performed. For (A), original magnification × 60. Data shown come from three independent experiments. Statistical analysis was performed using the Student’s t-test.
