Ethidium bromide staining (upper panel) and Southern hybridization (lower panel) of a representative PCR using the nested orf 26 (KS330) primer set. The sensitivity curve (consisting of a known copy number of HHV-8 from BCP-1 inoculated into 2 μg of Jurkat cell DNA) shows the limit of detection to be 3 genome copies per reaction by the presence of bands at the predicted 160-bp position (arrow) by both ethidium staining and Southern hybridization. Numbers 1 through 14 represent patient samples and show scattered positives.