American Society of Hematology

Fig. 2.

$Fig. 2. Detection of murine fac-RED complexes in liver extracts. Using the indicated antibodies and protein A-agarose, mouse liver cytosolic or microsomal extracts (730 μg) were used to immunoprecipitate fac, and immune complexes were analyzed for the presence of RED by probing the immunoblot with anti-RED antibodies (left). Each subcellular fraction (50-μg aliquots) was also analyzed directly without prior IP. Conversely, immune complexes obtained by IP with anti-RED antibodies were analyzed for the presence of FAC by immunoblotting (right). After SDS-PAGE (10% gel for the left panel, 8% to 20% gradient gel for the right panel), immunoblots were probed with the antibodies indicated in the bottom of the figure.$

Detection of murine fac-RED complexes in liver extracts. Using the indicated antibodies and protein A-agarose, mouse liver cytosolic or microsomal extracts (730 μg) were used to immunoprecipitate fac, and immune complexes were analyzed for the presence of RED by probing the immunoblot with anti-RED antibodies (left). Each subcellular fraction (50-μg aliquots) was also analyzed directly without prior IP. Conversely, immune complexes obtained by IP with anti-RED antibodies were analyzed for the presence of FAC by immunoblotting (right). After SDS-PAGE (10% gel for the left panel, 8% to 20% gradient gel for the right panel), immunoblots were probed with the antibodies indicated in the bottom of the figure.

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