Fig. 3.
Localization of the RED-binding domain of FAC to the amino terminal region. Carboxy-terminal truncated fragments of FAC (residues remaining indicated as subscripts) fused to the constant region of the human IgG1 heavy chain were coexpressed with full-length RED in COS-1 cells. Protein interactions were detected by IP of 35S-labeled lysates with protein A-agarose beads, followed by SDS-PAGE and autoradiography. The lower panel shows unlabeled lysates analyzed by immunoblotting with anti-RED antibody.
