Kinetics of mobilization of PBMC, HSC, and FC under treatment with FL alone (▴), G-CSF alone (◊), FL + G-CSF (□), or carrier (—-). FL (10 μg/mouse) was injected SC for 10 days and G-CSF (7.5 μg/mouse) from day 4 to 10. (A) PB was obtained daily and PBMC were counted. The percentage of HSC (lineage⁻/Sca-1⁺/c-kit⁺) and FC (CD8⁺/βTCR⁻/γδTCR⁻) was analyzed by flow cytometry, and absolute numbers of (B) HSC and (C) FC were calculated based on individual PBMC counts. Results represent the mean (SEM) of two different experiments (n = 5 per group). PBMC or absolute numbers of FC and HSC that differed significantly from controls are marked (* P < .005 or ** P < .0005).