Assessment of long-term engraftment of mobilized HSC and FC by three-color flow cytometry. PB was obtained from lethally irradiated B10 mice (H-2K^b) 6 months after reconstitution with PBMC from growth factor–treated B10.BR mice (H-2K^k) and stained with lineage- and donor-specific MoAbs. Unmanipulated B10 and B10.BR mice served as controls (data not shown). Figure shows results of a representative long-term surviving chimera. (A) Lymphocytes (R1) and granulocytes/macrophages (R2) were gated based on forward and side scatter. Engraftment of multiple donor derived cell lines including (B) B-cells, (C) T cells, (D) NK cells, (E) granulocytes, and (F) macrophages were detectable 6 months after transplantation indicating HSC engraftment.