Fig. 4.
Fig. 4. Immune deficiency does not develop in BDF1 recipients transplanted with CD28−/− donor cells. Splenocytes from CD28+/+ wild type, CD28−/− deficient, normal BDF1 mice, and BDF1 recipients of CD28+/+ or CD28−/− cells 2, 15, and 45 days after transplantation were cultured for 24 hours with ConA; and IL-2 in the supernatants was measured by using a sandwich enzyme-linked immunosorbent assay (ELISA) technique. IL-2 concentrations were calculated with reference to standard curves. Data represent the average +/− 1 SD of three mice per group analyzed separately for BDF1 recipients of CD28+/+ or CD28−/− cells. Data on CD28+/+, CD28−/− and normal BDF1 mice represent the study of one mouse at each time point. *Recipients of CD28+/+ donor cells died before day 45.

Immune deficiency does not develop in BDF1 recipients transplanted with CD28−/− donor cells. Splenocytes from CD28+/+ wild type, CD28−/− deficient, normal BDF1 mice, and BDF1 recipients of CD28+/+ or CD28−/− cells 2, 15, and 45 days after transplantation were cultured for 24 hours with ConA; and IL-2 in the supernatants was measured by using a sandwich enzyme-linked immunosorbent assay (ELISA) technique. IL-2 concentrations were calculated with reference to standard curves. Data represent the average +/− 1 SD of three mice per group analyzed separately for BDF1 recipients of CD28+/+ or CD28−/− cells. Data on CD28+/+, CD28−/− and normal BDF1 mice represent the study of one mouse at each time point. *Recipients of CD28+/+ donor cells died before day 45.

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