Biological activity of Wnt genes on hematopoietic stem/progenitor cells. Results of methylcellulose colony formation (A through C) and CD34 phenotypic analysis (D) are shown. Colony numbers (A through C) are mean values with standard deviation bars from duplicate plates. A total of three tissues were examined. Percentage CD34⁺ cells is the mean of two experiments with standard deviation bars. The cocultures represented are CV-1 cells transduced with the MSCVneoEB vector (CV1), CV-1 cells transduced with each of theWnt genes (CV1/5A, CV1/2B, and CV1/10B), an equal mixture of CV-1 cells transduced with each of the Wnt genes (CV1/5A/2B/10B), CV-1 cells transduced with the MSCVneoEB vector and 100 ng/mL of recombinant human SCF added to the culture medium (CV1 + rhSCF), and CV-1 cells transduced with the MSCVneoEB vector and 100 ng/mL of recombinant human IL-3 added to the culture medium (CV1 + rhIL-3).