Evaluation of fibrinogen (fg) binding in flow cytometry (A) and with ¹²⁵I-fibrinogen (B) at various concentrations of c7E3 without washout procedure. (A) The percentage of platelets binding fibrinogen was assessed by an FITC-labeled polyclonal chicken antifibrinogen antibody in flow cytometry. (B)¹²⁵I-fibrinogen was determined as described in Materials and Methods in relation to the maximum binding by stimulation of platelets with 20 μmol/L ADP. The MoAb P2 (20 μg/mL) was preincubated for 10 minutes at room temperature. Results are depicted as the mean of five determinations ± standard deviation. Using the unpaired Student’s t-test, P values less than .01 were obtained for the comparisons between no addition of c7E3 and the additions of 0.01 and 0.1 μg/mL c7E3.