Immunohistochemical and/or immunofluorescent staining analysis for vWF and albumin antigens and for LEA lectin in the lung and brain. Sections of murine lung (A and B) and brain (C, D, and E) were analyzed for vWF antigen (A, B, and C), for albumin (D), and for LEA lectin (E). Staining was visualized by immunofluorescence (A, C, and D) or regular immunohistochemistry (B and E). Arrows denote larger vessels, whereas arrowheads denote microvascular ECs surrounding aveoli in the lung (B) or present in the brain (C, D, and E). Original magnifications: for (A), (C), (D), and (E), ×250; for (B), ×1,000.