Inhibition of HIV-1_BZ167 replication by alloantigen-stimulated cell lines. (A) PHA blasts were infected with 859 TCID₅₀/10⁵ cells, as described in Materials and Methods section. Infected PHA blasts were cultured in the absence (□) or presence of an alloantigen-stimulated cell line derived from the same cells donor as the PHA blasts (autologous, •) or an alloantigen-stimulated cell line derived from an unrelated donor (heterologous, ▴). HIV-1 p24 core antigen was determined by ELISA. The results represent means of triplicate cultures. (B) HIV-1_BZ167–infected PHA blasts (172 TCID₅₀/10⁵ cells) were incubated in the absence or presence of an alloantigen-stimulated cell line before and after depletion of CD4⁺ T cells, using anti-CD4–coated magnetic beads. Results are expressed as p24 antigen production from one experiment determined by ELISA.