Analysis of CTL responses of alloantigen-stimulated cells using a standard ⁵¹Cr release assay. (A) Uninfected (□) or HIV-1_BZ167–infected (⧫) blasts were used as targets. The targets were incubated with the effector cells at the indicated E:T ratios for 4 hours. The results are expressed as percent specific lysis as described in Materials and Methods. (B) EBV-transformed B-cell lines from the same donor as the alloantigen-stimulated cell line were used as targets. Targets were pulsed overnight with the HIV-1 envelope peptides T1, T2, Th4.1, P18MN, P18 IIIB (5 μmol/L) and then incubated with the alloantigen-stimulated cell line for 4 hours. The results are represented as percent specific lysis observed at an E:T ratio of 60:1.