Effect of supernatants from an alloantigen-stimulated cell line on HIV-1 infection. (A) PHA blasts were infected with HIV-1BZ167 (172 TCID50/105 cells) and cultured in the absence (□) or the presence of supernatants that were obtained 2 (•), 4 (▴), and 7 days (▪) after cell line stimulation with allogeneic cells. The results are expressed as mean of triplicate cultures and are representative of one of two independent experiments. (B) PHA blasts were infected with HIV-1BZ167(172 TCID50/105 cells) and cultured with different concentrations (vol/vol) of allostimulated cell line–derived supernatant collected 4 days after stimulation. p24 antigen production was assayed at 3 days postinfection by ELISA. The results are expressed as mean of triplicate cultures using a representative supernatant. (C) Effect of allostimulated cell line supernatant (1:2 dilution) on viral replication in the HIV-1MN chronically infected H9 cell line after 3 days of coculture. p24 antigen production was determined by ELISA. Results represent means ± SEM of three different experiments.
